University of South Alabaa-8 Jump to... Puriodic Table stion 18 cf 20 A linear p

Question

University of South Alabaa-8 Jump to... Puriodic Table stion 18 cf 20 A linear piece of DNA was broken into random, overlapping fragments and each fragment was then Mp sequenced. The sequence of each fragment is shown below, listed in a random order. 5-ATCAAAAGC-3 5-TCAAAAGCATAGAGGTACC-3 5-GAGGTACCCTTC-3 5-CTATCAAAAGCAT-3 5-AAAAGCATAGAGGTACC-3 5-AAGCATAGAGGTACC-3 Using the averlapping regions shared between sequences, create a contig sequence of the original fragment by placing the sequence that is closest to the 5 end first and moving towards the 3' end. 5 end 3 end 5-AAAAGCATAGAGGTACC-3 5-AAGCATAGAGGTACC 5-ATCAAAAGC3 5-GAGGTACCCTTC-3 O Previous Check Answer O Next Hint

Explanation / Answer

Contigs are contiguous set of overlapping DNA sequence reads obtained from the Sequencing of DNA.

Below are the Original fragment obtained by overlapping the sequence read. I have highlighted the sequence region which are overlapping between the sequence and are continuous.

5’ – CTATCAAAAGCAT – 3’

5’ – TCAAAAGCATAGAGGTACC – 3’

5’ – GAGGTACCCTTC – 3’

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