The graph to the right shows a diauxic growth curve of E. coli grown in the pres

Question

The graph to the right shows a diauxic growth curve of E. coli grown in the presence of glucos and lactose. Diagram the status of the lac operon during stage IIl using the diagram below Which proteins (CAP Lacl) would be binding (or not) and where would they bind and what small molecules are required? Select one a. None of the above b. CAP/CAMP would bind to CAP site and Lac I would NOT be bound to 01/02 c. CAP would NOT bind to CAP site and Lac I would NOT be bound to 01/02 d. CAP would NOT bind to CAP site and and Laci would be bound to 01/02 e. CAP/CAMP would bind to CAP site and Lac I would be bound to 01/02 o

Explanation / Answer

The given graph is a Diauxic growth curve. In the media both glucose and lactose sugar are present.

At the end of stage III, there will not be any glucose in the media. As a result of it, adenyl cyclase will start converting ATP into cAMP. cAMP then make a complex with the CAP and bind at CAP site on the lac operon.

Since lactose is already present in the media. So it will bind to LacI and prevents LacI binding to O1/O2.

Hence Option B is the correct answer

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