In this experiment, poor cutting gives a 7kb fragment of DNA, while fragments of

Question

In this experiment, poor cutting gives a 7kb fragment of DNA, while fragments of 4kb and 3kb indicate good cutting of the DNA. The results are indicated below:

Construct                   DNA fragments observed    Chromatin structure

                                                                                    (tight, loose or intermediate)

g                                  4kb, 3kb

h and i                         7kb, 4kb, 3kb

j                                   7kb     

k                                  4kb, 3kb

d. Complete the table above to indicate the chromatin structure implied for each construct.

(4 pts)

e. If one of the Hippogenin or Griffogenin transcription factors modifies chromatin structure, whereas the other attracts RNA polymerase II, indicate which factor has which activity. Briefly explain your answer.

(1 pt)

Explanation / Answer

d. g - loose
h and I = Intermediate
j = Tight
K = loose

g and K have loose conformation of chromatin structure so it can be cut by restriction enzymes easily.
h and I are in intermediate state that why both full and digeated DNA present.
j is a tight conformation of chromatin structure so not able to digest by the enzymes.

e. In the presence of acetylated histones (HAT mediated) and absence of methylase (HMT) activity, chromatin is loosely packaged. Here Transcription factor unwind the chromatin structure and brings loosely packed.

RNA polymerase II brinds down a transcription.

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