3. Which of the following experimental observations would not support the chemio

Question

3. Which of the following experimental observations would not support the chemiosmotic model of oxidative phos- phorylation? A. If mitochondrial membranes are ruptured, oxidative phosphorylation cannot occur. B. Raising the pH of the fluid in the intermembrane space results in ATP synthesis in the matrix. C. Transfer of electrons through the respiratory chain results in formation of a proton gradient across the inner mitochondrial membrane. D. The orientation of the enzyme complexes of the electron transfer chain results in a unidirectional flow of H E. Radioactively labeled inorganic phosphate is incorporated into cytosolic ATP only in the presence of an H gradient across the inner mitochondrial membrane. 4. The proton-motive force generated by the electron transfer chain: A. includes a pH-gradient component. B. includes an electrical-potential-gradient component. C. is used for active transport processes. D. is used to synthesize ATP. E. has all of the above characteristics.

Explanation / Answer

3. Raising the pH of the fluid in the intermembrane space result in ATP synthesis in matrix.

Because intermembrane space has to decrease the pH - increase H+ ions here then it transport to Matrix via F0 to make PMF to synthesis ATP.

4. E. Has all of the above characteristics

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