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If r you wanted to isolate a copy of the gene that codes for a protein found in

ID: 253064 • Letter: I

Question

If r you wanted to isolate a copy of the gene that codes for a protein found in the stomach, could cheek cells? Explain your reasoning. The protease used in this procedure functions best at 50°C. Would you expect this enzyme to be isolated from Escherichia coli bacteria? Explain your answer. Hint: Where does E. coli live Meat tenderizer is often used to tenderize tough pieces of meat, like steak. Knowing that steak is made of p muscle tissue from cows, can you think of an explanation for how meat tenderizer works?

Explanation / Answer

1. The gene coding for a stomach protein is found in the genomic DNA inside a cheek cell. even though the cheek cell will not make the messenger RNA of the gene for the stomach protein. Stomach protein genes are expressed only in the stomach.

2. No. E. coli lives in our gut and grows around our body temperature that is 37°C.The optimal temperature of protease enzyme is 50°C. so the protease was isolated from an organism that lives near that temperature.

3. meat tenderizers include papain, which is a protease. The protease works by breaking down the protein molecules. Protease also works By partially deteriorating some of the proteins, the tough meat is made softer and more tender.

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