In a typical two-dimensional gel electrophoresis experiment, proteins extracted

Question

In a typical two-dimensional gel electrophoresis experiment, proteins extracted from two individuals were compared for evidence of differential protein expression. Based on the analysis of the two patterns of spots (seen below), what is a possible explanation for the difference between the two spots highlighted below? 250 250 Mass Mass (kDa) · | (kDa) 25 25 4 10 4 10 pH pH Person A Person B Select one: a. Person B has a deletion in the gene that expresses the protein of interest b. Expression of the protein of interest is lower in Person B compared to Person A c. There is a mutation-causing substitution in the gene that expresses the protein of interest d. Expression of the protein of interest is higher in Person B compared to Person A e. Person B has an insertion in the gene that expresses the protein of interest o

Explanation / Answer

According to the given ,comparison of bands in gel electrophoresis.Expression of the protein of interest is higher in person B compared to person A as in band of person 1 exhibit less molecular weight(in kDa) protein than person B. It is possible only when their is increase in the expression of protein.Thus person B has more expression of Protein in gel electrophoresis than A.

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