0002-E0 hwf1-1 (Compatibility Mode Layout References Mailings Review View AaBbCc

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0002-E0 hwf1-1 (Compatibility Mode Layout References Mailings Review View AaBbCcDdEeAaBbc abe X2 x2 Normal No Spa 3832 E01-Homework week #11 1. You've recently discovered the sequences for two alleles for the mouse gene XCD, which you have creatively named XCD and XCD. This gene is believed to be involved in fat metabolism, somehow, but you haven't successfully been able to isolate the protein yet. You'we also noticed that your mutant grey coat mice tend to be much leaner and muscular than those with the standard white coat. So for your pilot descriptive experiment with this gene, you'd like to determine if one coat color is more likely to have one allele over another. You're going to explore this hypothesils using a Southern blot a) Why is a Southern blot the better choice than a Western blot at this point of knowledge about the gene? (1-2 sentences) b) You've already isolated and purified DNA samples from both grey and white mice and have your gel set ups ready to go. What else do you need to do prepare for your Southern blot? c) Your results are presented here for both XCD and XCD alleles. Based on this pilot information, do you think it's likely that different alleles for the XCD gene could contribute to leanness/body wide energy balance? Explain your answer. (2-3 sentences) XCD1 XCD2 white English (US)

Explanation / Answer

a) Southern blot is the molecular biology technique which is used for the identification of particular DNA from the mixture of other similar molecules. Whereas, western-blotting is used for the identification of the protein.

Here in this experiment, we are analyzing the gene, we will prefer southern blot over western blot

b) A radioactive/non-radioactive probe has to be prepared which binds to XCD1 and XCD2 genes on the membrane, which is required for the identification of these genes on the membrane.

c) Yes, different alleles for the XCD gene contribute to leanness/bodyweight energy balance

As we can see from the southern blots, the XCD1 allele is expressed in mutant grey coat mice but not in white coat mice (left blot).

The XCD2 allele is expressed only in white coat mice but not in grey coat mice (right blot).

So XCD1 allele is responsible for leanness/body weight energy balance in mutant grey coat mice, while XCD2 allele contribute to normal White coat mice

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