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Name: BIO 2710: Diagnostics worksheet Instructions: Match each of the diagnostic

ID: 252302 • Letter: N

Question

Name: BIO 2710: Diagnostics worksheet Instructions: Match each of the diagnostic procedures below with the cotrect method, above. used more than once, or not at all. Answers may be Methods: A. PCR B. Western blot C. selective media D. ELVEL?SA F. Microscopic examination G. MIC H. Biochemical tests L. Anacrobic culturing . Malaria is diagnosed,in part,by viewing the mularia parasite on a stained blood smear from a 2. A fecal culture grown on Salmonella Shigella Agar, a medium where growth indicates that the 3. Bacteria of the genus Bactrmides can commonly cause abdominal infections, and must be grown in 4. The catalase test differentiates between pathogens of the genus Stapbylocacas and Stpococs patient enteric (gastrointestinal) pathogen in question is from either the Salmoneila or Shigella genus. oxygen-free conditions based on whether or not they possess (Stap) or do not possess (Srmp) the enzyme catalase In the third trimester of pregnancy, the birth canal is swabbed and tested for a 6. An assay to detect leptospirosis, caused by bacteria from the genus Leptojpina, involves misxing 7. Lyme disease is diagnosed, in part, by running Lyme disease proteins on a gel, transferring the gel to treptoccs Because the patient will be treated with antibiotics if the test is positive, Steplecocs to first, second, and third-line antibiotics is also determined. The test is read as positive or negative depending on whether the patient serum causes the Leptapina to clump. a membrane, and incubating the membrane with patient serum. If the patient has been exposed to the Lyme disease pathogen, they will have antibodies that bind to the membrane. 8. Tuberculosis can be diagnosed, in part, by the presence of Acid-fast bacteria in patient sputum 9. Tuberculosis can be diagnosed, in part, by amplification of DNA sequences specific to Mjyobaterin t0. HIV infection can be diagnosed, in part, by conjugating a known HIV antigen to a 96-well plate, wberalosis from patient sputum. dding patient serum to the plate, and adding a labeled antibody for human IgG.

Explanation / Answer

1) microscopic examination

2) selective media

3) anaerobic culturing

4) biochemical tests

5)

6) agglutination

7) ELISA test

8) selective media

9) PCR

10) ELISA test

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