Which of the double-stranded DNA sequences below will require more heat to separ

Question

Which of the double-stranded DNA sequences below will require more heat to separate the two strands (select Option A or Option B)? (2) Why is more heat is required to separate the strands? Option A 5’-GGACTCCCGGGTGAG-3’ 3’-CCTGAGGGCCCACTC-5’ Option B 5’-AACTGTTTAAAGACA-3’ 3’-TTGACAAATTTCTGT Which of the double-stranded DNA sequences below will require more heat to separate the two strands (select Option A or Option B)? (2) Why is more heat is required to separate the strands? Option A 5’-GGACTCCCGGGTGAG-3’ 3’-CCTGAGGGCCCACTC-5’ Option B 5’-AACTGTTTAAAGACA-3’ 3’-TTGACAAATTTCTGT Which of the double-stranded DNA sequences below will require more heat to separate the two strands (select Option A or Option B)? (2) Why is more heat is required to separate the strands? Option A 5’-GGACTCCCGGGTGAG-3’ 3’-CCTGAGGGCCCACTC-5’ Option B 5’-AACTGTTTAAAGACA-3’ 3’-TTGACAAATTTCTGT

Explanation / Answer

The correct answer is option A because it has more GC base pairs in it. There are three hydrogen bonds between G and C.

A and T has two double bonds between them. So, GC base pairs require more heat to break those bonds then AT base pair and since option B has more AT base pairs than option A, it would require less heat.

So, option A would require more heat to separate the two strands.

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