1. You have been given a 9 residue peptide of biological interest to study. You

Question

1. You have been given a 9 residue peptide of biological interest to study. You determine that the first thing you need to know is the sequence. Given below is the data you need to determine the sequence. What is it? To receive full credit you need to indicate what you learned from each step.

Composition is: Trp, Arg, Glu, Ser, Lys, Ala, Met, Phe, Val

A. Treatment with Dansyl Choride yielded a dansylated Glutamate


B. Treatment with carboxypeptidase yielded a Ser

C. Treatment with CNBr give a hexapeptide (Val, Glu, Phe, Trp, Met, Arg) and a tripeptide of composition (Lys, Ala, Ser)

D. Treatment with trypsin which yielded a dipeptide of sequence Ala-Ser, a tripeptide (Val, Glu, Arg) and a tetrapeptide (Trp, Phe, Lys, Met)

E. Treatment of the entire nanopeptide with Chymotrypsin gave two tetrapeptides (Glu, Phe, Arg, Val)(Lys, Ser, Ala, Met) and a free Trp

What is the sequence?

Explanation / Answer

C. Treatment with CNBr give a hexapeptide (Val, Glu, Phe, Trp, Met, Arg) and a tripeptide of composition (Lys, Ala, Ser)

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