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2. In phage crosses or mixed infections recombination frequency is calculated as

ID: 23737 • Letter: 2

Question

2. In phage crosses or mixed infections recombination frequency is calculated as the sum of recombinant plaques divided by the total number of plaques. To estimate very small recombination frequencies Benzer devised a system in which only recombinant phage could grow and form plaques. The total number of plaques (recombinant and nonrecombinant ) was estimated by plating a greater dilution on a permissive bacterial strain. If 103 recombinant plaques are counted and the total number of plaques is 10,463, the recombinant frequency is

a. 0.23 b. 0.86 c. 0.32 d. 0.0098 e. 0.103

Explanation / Answer

b. 0.86

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