Which of the following set-up critera must be true to perform successful phase c

ID: 2306094 • Letter: W

Question

Which of the following set-up critera must be true to perform successful phase contrast microscopy?

A.The phase plate must be conjugate to the image plane.

B.The condenser annulus and the phase plate must be conjugate to each other.

C.The image of the annulus must be focused in the specimen plane.

D.The background light must be shifted by ? radians relative to the diffracted light.

E.The background light must be shifted so that it is either in phase or 180o out of phase relative to the diffracted light.

E.The background light must be shifted so that it is either in phase or 180o out of phase relative to the diffracted light.

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