You are performing an in vitro experiment in which you will expose a material yo

Question

You are performing an in vitro experiment in which you will expose a material you are
considering for a medical device to synovial fluid, which contains the proteins albumin,
transferrin, and IgM at concentrations of 5, 0.5, and 0.05 mg/ml, respectively. Each of these
components has a particular affinity for your material, with IgM being the highest and
albumin being the lowest.
a. Describe the kinetics of protein adsorption to your material and how the surface
concentration of each protein will change with time.
b. Subsequently in the experiment, more albumin is added to the synovial fluid. What effect
will this have on the proteins adsorbed on the surface?

Explanation / Answer

Describe the kinetics of protein adsorption to your material and how the surface concentration of each protein will change with time.

The kinetics of the above concentration of synovial fluid proteins such as albumin,
transferrin, and IgM at concentrations of 5, 0.5, and 0.05 mg/ml, respectively. The protein adsorption is variable as per surface affinity (Vroman effect), mainly based on concentration in which initially albumin is going to adsorb (finally diffusion) followed by adsorption of transferrin, and IgM due to greater surface affinity on to the adsorbent.

Subsequently in the experiment, more albumin is added to the synovial fluid. What effect will this have on the proteins adsorbed on the surface?

High addition of albumin is leading to minimal surface adsorption on the material surface due to low effect in case IgM has already undergone adsorption to material surface

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