Molecular biology: Explain the result of this experiment in detail please. How d

Question

Molecular biology: Explain the result of this experiment in detail please.

How do you explain the percentage differences in each (6) rows.

For example, why given uridine will result in 53%CMP and 47%UMP, why is there a different? Why there is a great different in next row with Cytidine?

5) In a paper from 1972 Plageman described an experiment where he fed yeast a single radioactive nucleoside. The cells took up these nucleosides and added them to their pool of RNA nucleotides When he looked at which nucleotides within the RNA that was produced in these cells was radioactive he found the following results. means that no appreciable levels of radioactivity were found in that nucleotide) Radioactive nucleoside Percentage of total radioactivity found in the RNA of the yeast GMP CMP AMP 47% Uridine 53% 5% 95% Cytidine 93% 7% Guanosine 49% 51% inosine 35% 65% Adenosine Given what we talked about in class explain the results above.

Explanation / Answer

Uracil is incorporated in to nucliec acid as UTP and CTP. Hence, a number of enzymaic reactions must ensue between the aptake of uracil in the cell and its use for transcription. Although the incorporation of radioactive uracil appears linear from early experiments as you mentioned Plageman 1972, this is infact a delusion arising from counteravailing effects of two linear events:

1. convex labelling profiles of unstable RNAs: the fact that unstable RNAs are synthesized and degraded (the amount of radiactivity in unstable RNAs after a short pulse is out of the proportation to their representation in the bulk RNA, for example, Poly A mRNA makes up 3-5 % of total RNA but as much as 30 % of a 2 min pulse).

2. the approach to maximum value of specific activities of UTPs and CTPs which can take up to 15 min.

Same is true to other nuclsosides.

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