e The d Expressios vectors available 21. advantages are may for in above reasons

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Explanation / Answer

21. D. all above are advantages of insects-

a. insects are easier to grow than mammalian cell lines- The cost is very high of growing mammalian cell cultures so research is being carried out to produce proteins in insect cells

b. baculovirus vectors will kill the insect host and produce large amounts of the protein - Using baculovirus expression system for protein expression and purification takes ~ 2 months which is currently the fastest method to obtain mg amounts of soluble protein. This need cannot be met by using mammalian expression.

c. shuttle vectors can replicate as plasmids in E.coli- These vectors can be easily replicated (gene amplification) and manipulated in E. coli then in a slower system like yeast which is difficult to use

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