A temperature sensitive e.coli mutant is discoved that accumulates okazaki fragm

Question

A temperature sensitive e.coli mutant is discoved that accumulates okazaki fragments and doesn't complete replication when grown at elevated temperatures. Which enzyme and/or enzyme function do you suspect has been mutated? List 4 of the enzymes required for replication and briefly describe their function. Briefly describe the different strategies used by prokaryotes vs. eukaryotes to codense their genome. In Saccharomyces cerevisae a cross involving three linked genes is made (ABC times abc). Among 200 asci, one is found to contain the spores ABC, abc, Abc, and abC. What is unusual about this ascus? What event could explain this ascus?

Explanation / Answer

Question No.1

Question No.2

Four enzymes required for the replication are:

Family

Types of DNA polymerase

Species

Examples

A

Replicative and Repair Polymerases

Eukaryotic and Prokaryotic

T7 DNA polymerase, Pol I, and DNA Polymerase

B

Replicative and Repair Polymerases

Eukaryotic and Prokaryotic

Pol II, Pol B, Pol , Pol , , and

C

Replicative Polymerases

Prokaryotic

Pol III

X

Replicative and Repair Polymerases

Eukaryotic

Pol , Pol , Pol , Pol , and Terminal deoxynucleotidyl transferase

Y

Replicative and Repair Polymerases

Eukaryotic and Prokaryotic

Pol (iota), Pol (kappa), Pol (eta), Pol IV, and Pol V

Family

Types of DNA polymerase

Species

Examples

A

Replicative and Repair Polymerases

Eukaryotic and Prokaryotic

T7 DNA polymerase, Pol I, and DNA Polymerase

B

Replicative and Repair Polymerases

Eukaryotic and Prokaryotic

Pol II, Pol B, Pol , Pol , , and

C

Replicative Polymerases

Prokaryotic

Pol III

X

Replicative and Repair Polymerases

Eukaryotic

Pol , Pol , Pol , Pol , and Terminal deoxynucleotidyl transferase

Y

Replicative and Repair Polymerases

Eukaryotic and Prokaryotic

Pol (iota), Pol (kappa), Pol (eta), Pol IV, and Pol V

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