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Lao Qua B generation time 40 you be your calculated number of 2. Aher generation

ID: 225381 • Letter: L

Question

Lao Qua B generation time 40 you be your calculated number of 2. Aher generation per hour. change in the geneatontmeot 20 minutes ane two tactors that could have 3 A cuture unknown many eelis were present the original sample? Please use netason Yeu xpect on the plate plated intead of01 mL One thousand mioolters Staphylocorous broth plated onto agar and incubated are in the originalboth? Express the Spectrophetomety commonly used to ack bacter growth. disadvantage tothis technique when tracking growth a bl Which other method did we use Draw an appropriate growth exponentially growing Ecoloristarsferred G. You estimate that your ourue contains about2xtor Tha conoennaionistoedense and you omode to dane your culture before oreating pour plates of the appropriate daMons would prepare to obtain statistically significant valuma Please provide an approp d yo" your pour ml in the orginal cuture Determine the number of bacterial too many 500 128 12 0 were aven a tbe containing 10 ml of frozen Staphylococcus epidemmids cuture known to contain s3R B Suppose you You were c tor 15 min, andthen prepare tour 10 deutors How many colonies would you expect to find on the plane?(9 STOP HERE CREDIT OSOLATED COLONIES FLOwsHEET Satisfactory Needs some improvement Needs much improvement LAB REPORT: Satisfactory (15) Needs some improvement (10) Needs much improvement Nov 8 016 holes and safety glasses Shared with Shoebox

Explanation / Answer

Ans 1 A . The generation time for pseusdomonas is 40 minutes. And the culture is incubates for 4 hours or 240 minutes. Which makes 6 cycles so the number of generations will be 7.

B. If you have started with initial cells 2 ×103 , so after 4 hours the number of cells in the culture will be 1.28 × 105 cells

C. The culture cells can be plated and then CFU ( colony forming unit) counting can be done to know the number of cells. If the number match with the calculations , the latter is probably correct.