can you help me answer 1-5 based on the chart to the right. please Testing Your

Question

can you help me answer 1-5 based on the chart to the right. please

Testing Your Knowledge I. Study the gel to the right. DNA fragments were placed into the wells at the bottom, and then the DNA fragments moved up when an electric current was applied, Similar-sized DNA fragments moved at the same rate, forming the bands shown. Knowing that DNA is negatively charged, would the negative pole be closer to or farther from the "wells" where the DNA was loaded? 2. Would the DNA separate into usable bands if the negative pole and positive pole were reversed, but the gel remained in the same position? 3. Are the smallest DNA fragments closer to or farther from the "wells" where the DNA was loaded? 4. If a DNA molecule 2400 base-pairs long was cut into four fragments that are 200, 450, 740, and 1010 base pairs, which fragment would move the farthest with electrophoresis? 5. Which is more important in determining the speed ofa fragment length or fragment weight?

Explanation / Answer

Q1.Since DNA is negatively charged and it will move towards positive charge, hence close to wells it will be negative charge and farther from the well will be positive charge.

According to chegg minimum protocol guidelines I have to answer just the first full question but as a courtesy i will answer few more.

Q2 No if the polarity is reversed dna will get out of wells into the electrolyte solution and hence bands wont be resolved .

Q3. Smaller fragments will move more quickly and hence will be farthest from the well, where as bigger heavier particles will have less mobility and hence will lag behind smaller particles closer to well.

Hope it helps .... Regards

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