In qPCR, the relationship between the threshold cycle (CT) and template DNA conc
ID: 225004 • Letter: I
Question
In qPCR, the relationship between the threshold cycle (CT) and template DNA concentration is
the higher the CT value, the higher the concentration of template DNA in your sample
the higher the CT value, the lower the concentration of template DNA in your sample
the lower the CT value, the higher the concentration of template DNA in your sample
the lower the CT value, the lower the concentration of template DNA in your sample
both B and C
both A and D (NOT THIS ONE)
None of the above. There is no relationship between the CT value and template concentration.
Explanation / Answer
The Ct value increases with a decreasing amount of template.
So answer is both B and C
In B and C it they are inversely proportional.
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