Nucleotides are required for cell growth and replication. You are given a mutant
ID: 224282 • Letter: N
Question
Nucleotides are required for cell growth and replication. You are given a mutant human cell line in which a key enzyme necessary for making adenine nucleotide is mutated (a small deletion in the gene), hence it can't make this nucleotide. You can grow this mutant cell line if you provide adenine nucleotide in the medium. You also have a wild type human cell line that can make adenine nucleotide. Design an experiment using these two cell lines to demonstrate that DNA is the genetic material in human cells.Explanation / Answer
Nucleotides are indeed required for cell growth and replication since they are essential and integral part of the DNA. According to the information, the two available cell lines differ in the manner that one of tehm is unable to synthesize the nucleotide whereas the other one is able to synthesize the nucleotides. Thus, an experiment with objective to ascertain that DNA is the genetic material can be designed as follows:
Thus, the above given experiments will help in achieving the objective of the study.
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