..ooo T-Mobile LIE 12:41 PM 86% montclair instructure.com q1 You have the shown
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Question
..ooo T-Mobile LIE 12:41 PM 86% montclair instructure.com q1 You have the shown DNA strand. The restriction enzyme Mscl recognizes the consensus sequence, TGG CCA There is a second allele which contains a SNP at the location of the star. You design a probe to determine the presence of either allele for several individuals. The probe location is indicated by the box saying probe. In the gel image for Q1, indicate which of the following genotypes each individual is using one of the following descriptors: homozygous allele 1, homozygous allele 2, or heterozygous Individual 1? individual 2? individual 3? individual 4? individual 5?Explanation / Answer
Answer:
1. The probe will bind to the corresponding DNA and show up on the gel. In allele 1 containing the MscI restriction site, cleavage of the fragments will produce two fragments (250bp and 400bp), but the 250bp band will show up as the probe binds to it.
In allele 2, MscI will not cleave since the restriction site contains an SNP. So, the full fragment length of 650bp will show up in the gel.
Individual 1: Homozygous allele 2 (650bp band)
Individual 2: Homozygous allele 1 (250bp band)
Individual 3: Heterozygous
Individual 4:Homozygous allele 2 (650bp band)
Individual 5: Homozygous allele 1 (250bp band)
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