(20 points t al You are investigating how the transcription factor BLUE1 enters
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Question
(20 points t al You are investigating how the transcription factor BLUE1 enters into the nucleus to turn on gene transcription and expression. You introduce a green fluorescent protein tagged version of two importin GFP) into wildtype mutant cells deletions in cells (wt) and GFP tagged protein and compare it to the localization of a fluorescent molecule in (DAPI) that binds to DNA. Fluorescence is indicated by the shaded the diagram. DAPI BLUE1-GFP a. (4 points) Examine the illustration to the right. Given these data, is either WELLa or WELLB important for import of BLUE1 into Wt the nucleus? Explain how you know in 2 sentences or less. AWELLa AWELLB (4 points) You next want to test BLUE1-GFP localization in the absence of the Ran GTPase, but you are unable to create a Ran deletion mutant. Propose an deletion is for why WELLa or WELLA deletion is able to survive, but a Ran lethal.Explanation / Answer
Answer a) the mutant of well alpha is not able to import blue1 into the nucleus as the fluoroscent region is not shaded along with DAPI. This indicates that well alpha is important for import of BLUE1 but well beta is not.
Part 2: we are unable to create a RAN deletion mutant because it helps in transport in and out of the nucleus during interphase. Translocation of RNA and protein through nuclear pore complex is also done by this protein. It is important in the control of DNA synthesis and cell cycle progression.
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