micro CUNY an be safely handled which the fo respond Agents that can casuse dise

Question

micro CUNY an be safely handled which the fo respond Agents that can casuse diseases that do not icrobial therapy or to B. healthy adults e risks in healthy adults but not likely to spread throna Agen s that pose only moderat and readily rentable spread very easily use disease healthy adults that can munity though can be readily to healthy adults Agents not likely to pose a disease risk D. The magnifying lenses found in a light microscope are the ple and compound lenses Ar sim ocular and condenser lenses C. ocular and objective lenses D. condenser and objective lenses E- ocular, Objective and condenser lenses 3. The resolving power ofa microscope indicates A. the smallest size of an object that can be observed with the microscope B. the ability of the microscope to clearly separate two objects that are very C. the ability of the microscope to magnify an object D. both A and B are correct E. All of the above are correct 4. The instrument that regulates the amount of light reaching the slide on a micro A. the objective lens B. the ocular lens C. the diaphragm D. the coarse adjusting knob E. the side arm 5. Basic dyes have cationic chromophores. This means that the chromophor A. have positive charges B. have negative charges contain both positively and negatively charged particles D. have chromium ions E. are acidic 6. Dyes used for negative staining have A. cationic chromophores B. chromium ions C. anionic chromophores D. acid-fast ability E basic pH

Explanation / Answer

1. BSL3 corresponds to agents that causes diseases that do not respond to anti microbial therapy or vaccines even in healthy adults (A)
2. Ocular, objective and condenser lens are found in light microscope (E)

3.The resolving power of a microscope clearly defines the ability to clearly seprate two objects that are close (B)

4. Diaphragm controls the amount of light reaches the specimen (C)

5. Basic dyes have cationic chromophores are positively charged (A)

6.Dyes used in negative staining contains anionic chromophores (C)

7.Both gram staining and acid fast staining are differential staining techniques (E)

8.All appear gram positive by cell wall shrinking if we use strong alcohol in gram staining (B)

9.Oil in an immersion microscope decreases refraction (C)

10.Order of gram staining will be crystal violet, iodine, alcohol and safranin (B)

11.Acid fast stain uses carbol fuchsin as primary stain and methylene blue as counter stain and can also distinguishes small group of micro organisms. Hence both B and C are correct (E)

12.Schaeffer fulton endospore staining usually shows spores as green structure among the background because of malachite green as primary stain (A)

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