You have identified various tRNAs from a variety of hypothetical orgainisms. Giv

Question

You have identified various tRNAs from a variety of hypothetical orgainisms. Given the anticodon of the tRNA, determine if each tRNA individually could conform to the nearly universal genetic code. Assume that the tRNA in question can only be charged with one amino acid and it is the same amino acid everytime. If the tRNA is not intended to be charged with an amino acid, it can never be charged with an amino acid.

1. Anticodon: 3' A C U 5'

2. Anticodon: 3' U A I 5'

Could this tRNA conform to the nearly universal genetic code? yes or no and why, please

Explanation / Answer

Answer:

1. For anticodon: 3' ACU 5' the codon is 5'UGA3'. In the universal genetic code, this codes for a stop (chain terminating) codon. Since, there are no tRNA molecules with anticodons for a stop codon, therefore, tRNA with anticodon 3'-ACU-5' couldnot conform to the nearly universal genetic code

2. For anticodon 3' UAI 5', a tRNA can bind codon AUU, AUC and AUA which all code for isoleucine. Therefore this anticodon conforms to the universal genetic code. (I - Ionosine can base pair with A, C, U)

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