A mutant strain of E. coli is found that produces both B-galactosidase and Lacto

Question

A mutant strain of E. coli is found that produces both B-galactosidase and Lactose Permease constitutively (that is, whether lactose is present or not).

(a) What are two possible genotypes for this mutant?

(b) A second mutant is isolated that produces no active B-galactosidase at any time but produces Lactose Permease if lactose is present in the medium. What is the genotype of this mutant? Be sure to include all four appropriate genes in your genotype. (ie. LacY should be one of your genes you include)

A partial diploid is created from the mutants in parts (a) and (b): When lactose is absent, neither enzyme is made, and when lactose is present, both enzymes are made. What is the genotype of the mutant in part (a)?

Explanation / Answer

ANSWER:

a) The possible genotypes are WILD TYPEand SUPER REPRESSED

b) CONSTITUTIVE I-O+Z+Y+A+ Z,Y,A Z, Y, A

c) WILD TYPE I+(CAP)P+O+Z+Y+A+ Z, Y, A

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