The acute toxicity of a compound after intra-peritoneal injection was assessed i

Question

The acute toxicity of a compound after intra-peritoneal injection was assessed in species and the major organs examined for evidence of necrosis (+) or absence of necrosis(-): Discuss these results and suggest possible explanations for the results obtained. Describe briefly further experiments you would carry out to test your proposals. Groups of live rats were pre-treated with cither saline alone (control) or with phenobarbital (twice daily for four days) or with a single injection of 3-methylcholanthrene (24 hours before further treatment). The animals were then treated with the muscle relaxant zoxazolamine and the paralysis time and plasma half life (t_1/2) of zoxazolamine measured. The^a Mean plusminus standard error of the mean Explain these results and any additional experiments that might help test your proposals.

Explanation / Answer

Answer to second question:

1. Zoxazolamine is a muscle relaxant and and duration of zoxazolamine induced paralysis can be used as an indicator of drug clearance. In case of rats pretreated with phenobarbital or 3MC, the activity of the liver microsomal enzymes which metabolizes zoxazolamine increases.

Compared to control (untreated), rats treated with Phenobarbital hydroxylate Zoxazolamine to a greater extant and and results in its rapid clearance. This causes a decreases zoxazolamine duration of action which is reflected by a reduction in duration of paralysis.

In case of 3MC treated mice, the extent of induction of zoxazolamine hydroxylation is even more as compared to Phenobarbital leading to a much faster clearance and reduced paralysis time.

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