34) You have obtained a sample of DNA, and you transcribe mRNA from this DNA and

Question

34) You have obtained a sample of DNA, and you transcribe mRNA from this DNA and purify it. You then separate the two strands of the DNA and analyze the base composition of each strand and of the mRNA. You obtain the data shown in the table below. Which strand of the DNA is the coding strand, serving as a template for mRNA synthesis? DNA strand #1 DNA strand #2 mRNA 24.2 30.8 25.7 19.3 0 19.126.0 31.0 23.9 0 19.0 25.93002 a) Strand1 b) Strand 2 c) Both strands 1 and 2 d) Neither strand 1 nor 2 e) Too little information to tell

Explanation / Answer

Which strand of the DNA is the coding strand, serving as a template for mRNA synthesis?

A) Strand 1

Complementary base pairing is used to determine the base pairing. For two DNA strands: A pairs with T and G pairs with C. The A codes for U when making mRNA. Therefore, in strand 1, the A count most closely matching the % of U in mRNA strand that is our template strand. This is the strand 1.

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