1. You are working on an experiment in yeast and wish to know which polymerase t
ID: 220029 • Letter: 1
Question
1. You are working on an experiment in yeast and wish to know which polymerase transcribes your favorite gene. Your species cannot be transformed- so you can’t make mutations in the polymerases or alter the genome in any way.
a. Briefly describe how you could determine which polymerase transcribes your gene.
b. Describe the experimental results that would indicate it is transcribed by a Class I gene (2pts).
2. For each of the following: define it, for proteins provide their function. Remember, providing an example is not a definition (1 pt each).
PTEF-b
Gdown1
Nucleosome free region
Bromodomain
H2A.Z
H3.3
Explanation / Answer
Ans.1.(a)
Ans 1(b)
DNA sequences in specific gene promoters provides the code which can tell the level of specific genes to be transcribed. Code is mainly of three parts: core promoter, region proximal to the core promoter, and distant enhancer sequences. The core promoter sequence targets the assembly of distinct preinitiation complexes (PICs) which are composed of general transcription factors. Promoter-proximal regions and distant enhancer sequences directs binding of specific transcription factors which are called activators or repressors. Some co-regulators interacts directly with Pol II and GTFs, and influences the expression of gene. Others reorganize the nucleosomes and change the chromatin architecture of the gene this influences the transcription-factor associations and the status of transcription of Pol II.
Ans 2.
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