Use the following information to answer questions 10-12. Mutant organisms have b

Question

Use the following information to answer questions 10-12. Mutant organisms have been especially important in determining replication and other key biological processes. Especially important in this been temperature sensitive mutants which allow the organisms to grow normally at permissive temperatures and to exhibit the mutant phenotype at elevated or the restrictive temperatures. Such experiments were conducted with E coli mutants resulting in the observations described below. the mechanisms of DNA regard have 10. At the restrictive temperature mutant E. coli synthesized DNA just like the wild type organism except that there was an unusual accumulation of low molecular weight DNA This observation suggests a mutation in A) DNA polymerase III B) Topoisomerase C) DNA Primase D) DNA ligase E) RNA polymerase 11. At the restrictive temperature mutant E. coli was unabls to synthesize the short pieces of RNA that are associated with DNA replication. This observation suggests a mutation in A) DNA polymerase III B) DNA polymerase I C) DNA Primase D) DNA ligase E) RNA polymerase the restrictive temperature mutant E. coli was unable to remove RNA primers and the synthesis of Okazaki fragments. This observation suggests a mutation in NA polymerase III NA polymerase I DNA Primase ) DNA ligase E) RNA polymerase

Explanation / Answer

10. DNA ligase
The joining of DNA strands together by catalyzing the formation of a phosphodiester bond.

11. DNA primase
DNA primase is used to synthesize the primer to initiate the replication.

12. DNA polymerase I
DNA polymerase I has a 3'-5' exonuclease activity to remove the primers.

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