You have discovered a toad living in the subarctic near Churchill, Manitoba. As

Question

You have discovered a toad living in the subarctic near Churchill, Manitoba. As a research assistant you propose a study to characterize the winter acclimatization response. Select the study protocol that is most appropriate based on your knowledge of wood frogs (Rana sylvatica). Sample toads once per month over a year and measure blood Na and heat shock proteins Sample toads in August (control) and then prior to the predicted first frost, sample at a few time intervals over the period of below zero ambient temperatures. Measure blood oxygen levels. Sample toads once in August and once in December. Measure the tissue glycinin levels Sample toads several weeks prior to the predicted first frost and then over the time period where ambient temperatures fall below zero. Measure blood glucose, glycerol, adrenaline and the percent body water that is frozen.

Explanation / Answer

to characterizing the winter acclimatize response, we have to sample toads several weeks prior to the predicted first frost and then over the time period where ambient temperatures fall below zero. then measure blood glucose, glycerol, adrenaline and the percentage body water that is frozen.

option D is correct.

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