A graduate student in a laboratory that studies the bochemistry of cytochrome ox

Question

A graduate student in a laboratory that studies the bochemistry of cytochrome oxidase needs to purify a new batch of this enzyme for her experiments. In her lab this is usually done by first punifying mitochondria from rat liver and from there punilying the protein. The punfication usualy takes about a week and the yield is low The student comes up with a new strategy to oblain pure cytochrome ooxidase. She will use PCR to amplity the coding region for cytochrome oxidase from mitochondnial DNA The primer will also contain nucleotides that encode a 6Xhis tag that can coli induced and the protein punified is this strategy likely to work? O A. Yos-this is a very good strategy O B. Yes-but t would be better to use a yeast expression vector on an artificial chromosome O c No-this strategy is flawed

Explanation / Answer

Answer: Option B is correct.
Explanation: Rat is a eukaryotic organism. So, Cytochrome oxidase most likely contains introns or it may need posttranslational modifications for its function. If we express this protein in E coli, neither post-translational modifications nor splicing would occur. So, it is better to use eukaryotic expression system such as yeast.

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