This sketch shows a highly polymorphic Simple Sequence Repeat (SSR) region in th

Question

This sketch shows a highly polymorphic Simple Sequence Repeat (SSR) region in the genome. If you wanted to genotype individuals for copy number vaniations in this rogion, whore would you design PCR primers? AATG 5 6 8 9 O A Two primers complementary to the SSR sequences, pointing 5 33 O B. One primer complementary to the SSR sequence, one complementary to the adjacent sequence, pointing 53 towards each other OC. One primer complementary O D. Both primers complomentary to sequonces adacent to the SSR region, pointing 5 ? E. Both pr mers towards each other to the SSR sequence, one complementary to the adjacent sequence, pointing 53 away from each other 5>3 towards each other complomentary to sequences adjacent to the SSR region, pointing 5.>3 away from each other

Explanation / Answer

Answer: Option D is correct.

Explanation: To determine the SSR sequence, both primers must flank the polymorphic sequence. For a PCR product to be formed, both primers should face each other in 5'-3' direction.

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