A dominant wild-type allele D produces full enzyme function, but a recessive all

Question

A dominant wild-type allele D produces full enzyme function, but a recessive allele d1 produces no functional enzymatic action, and a recessive allele d2 produces reduced enzyme function. Western blot analysis of the proteins produced by organisms with different genotypes for this gene gives the results shown Part A What kind of a protein change might result in two western blot bands for organisms with the Dd2 genotype? The change in the protein encoded by d2 relative to wild type is either a change destabilization of secondary structure, or some combination of the three. The change in the protein encoded by d2 relative to wild type is either a change or some combination of the two. The change in the protein encoded by d2 relative to wild type is either a change combination of the two. The change in the protein encoded by d2 relative to wild type is either a reduction some combination of the three. in the expected folding of the protein, a shortening of protein length, in the surface charge, changes in the functionality and location of the active sites, GenotypeDD Dd, Dd dd, dd, dd, of molecular amphiphilicity and stability, changes in allosteric regulation, or some in molecular weight, a compaction in shape, a shift in charge to more negative, or Submit Request Answer

Explanation / Answer

The correct answer is (d)

From the western blot given in the picture, we understand that both the proteins coded by D and d2 are different as they are forming two seperate bands. Separation of the proteins in the western blot (or in PAGE) occurs due to a difference in electrophoretic mobilites (which is a function of length of the polypeptide chain and it's charge) while it is denatured. Thus structure of the protein does not come into play during western blotting as it is denatured anyway before performing the electrophoresis. Also as we can see the protein coded by d2 is more towards the anode, it can be understood that it's more negative relative to the protein coded by D.

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.