5\' TCAG TCATAATCCCAT GGAC GCG ATTATA GCTAGCTAGCTA TGTCAA 3\' Above is the seque

Question

5' TCAG TCATAATCCCAT GGAC GCG ATTATA GCTAGCTAGCTA TGTCAA 3'

Above is the sequence of a DNA strand from part of a bacterial gene. Use it to answer the following questions. (HINT: start by writing the strand shown above and then filling in the other strand, and its directionality, on paper)

Which side is the promoter on? Enter left or right for your answer

Is the strand you see going to be the coding or template strand during transcription?  (enter coding or template for your answer)

Which base is at the +1 position (transcription start site)? enter thymine, guanine, cytosine or adenine for your answer

Enter the sequence of the mRNA that will be transcribed, starting with the 5'end.

In the mRNA, locate the 5'UTR and 3'UTR. How many nucleotides are in the 5'UTR? (just enter the number of nucleotides, you do not need to enter the sequence)

Use the codon chart in your textbook (table15.1) to decipher the code. Enter the first 3 amino acids in the protein, in the correct order. (for example if you think they are all serine you'd enter it like this: serine,serine,serine )

enter the total number of amino acids this protein will have  

Explanation / Answer

1. Promoter is on the left side

2. The strand seen is a coding strand.

3. Adenine is at +1 transcription start site.

4.5'UCAGUCAUAAUCCCAUGGACGCGAUUAUAGCUAGCUAGCUAUGUCAA3'

5. 14 nucleotides are in 5' UTR.

6. Methionine, Aspertic acid, Alenine.

7. There will be 3 amino acids in the protein.

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