1 What is the role of restriction endonucleases in bacterial cells? a. to degrad
ID: 218746 • Letter: 1
Question
1 What is the role of restriction endonucleases in bacterial cells? a. to degrade the bacterial chromosome into small pieces during replication b. to degrade invading DNA at specific recognition sites c. to produce RNA primers for replication d. to assist in DNA replication Vectors are: a. molecules that degrade nucleic acids b. molecules that are able to covalently bond to and carry foreign DNA into cells c. molecules that protect host cells from invasion by foreign DNA d. none of the above 3 Which gene transfer technique involves a tiny needle which is used to inject DNA into a cell lacking that DNA sequence? a. electroporation b. liposome transfer C. microinjection d. particle bombardment In the process of cloning eukaryotic DNA into prokaryotic cells, the role of reverse transcriptase is to make a. double-stranded eukaryotic cDNA from mature mRNA b. mature mRNA from precursor mRNA c. bacterial DNA from eukaryotic DNA d. mRNA from DNA 5 Which of the following strategies would help to improve ease of purification? a. Adding signal sequences that causes secretion into the culture medium b. Adding molecules that helps the protein to refold and stay in solution c. adding a sequence coding for an affinity handle d. Both a and c e. All of the above 6 Which of the following is an example of a protein recovery process? a. Large scale chromatography b. electroporation c. amplification d. fermentation 7 If host cells are ampicillin sensitive and are plated on a medium containing ampicillin a. only cells that have not taken up the ampicillin resistant vector can grow b. only cells that have taken up the ampicillin resistant vector can grow c. all cells will grow d. ampicillin is inactivatedExplanation / Answer
AS PER CHEGG POLICY, PROCEEDING TO ANSWER ONLY THE FIRST 4 QUESTIONS / SUBQUESTIONS / PARTS / SUBPARTS, THAT IS, QUESTIONS 1 TO 4, UNLESS SPECIFIED TO DO SO BY THE QUESTION UPLOADER:
ANSWER 1
b. to degrade invading DNA at specific recognition sites
ANSWER 2
b. molecules that are able to covalently bond to and carry foreign DNA into cells
ANSWER 3
c. Microinjection
ANSWER 4
a. double-stranded eukaryotic cDNA from mature mRNA
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.