You’ve set up a test tube that contains all of the eukaryotic translational mach

Question

You’ve set up a test tube that contains all of the eukaryotic translational machinery (so, eukaryotic tRNAs, aminoacyl tRNA synthetases, and ribosomes) and some radioactively labeled amino acids. You throw some human Nbd gene mRNA into this tube and you find that you’ve produced a bunch of radioactive Nbd protein, which is great news for your new experiment. However, for your experiment, you also need some radioactive OMG protein, which is coded by the E. coli OMG gene.  If you threw in an mRNA OMG transcript isolated from E. coli cells, would you expect to see radioactive OMG protein? Why or why not? (2?3 sentences)  

Explanation / Answer

No, the prokaryotic protein wont be translated. As,eukaryotic mRNA contains a 5`cap and internal ribosome binding site which is responsible for recruiting translation machinery to the mRNA for translation. These sites are absent in prokaryotic mRNA besides that it contains Shine-Dalgarno sequence AGGAGG, which base pairs with the 3` end of 16S region of ribosomal unit and initiates translation. Due to this disparity in Ribosome binding site, translation of prokaryotic mRNA will not be initiated.

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