You have an enzyme, its substrate, and a small molecule. If you perform a reacti

Question

You have an enzyme, its substrate, and a small molecule. If you perform a reaction with the enzyme and substrate, you can detect lots of product after 5 minutes. If you perform a second reaction with all three, you fail to detect product even after 30 minutes. When you further analyze your second reaction, you determine that the substrate is bound to the enzyme. Why is no product produced when the substrate can bind the enzyme? The small molecule changes the AG of the reaction. The small molecule is an enzyme cofactor. ?The small molecule is a noncompetitive inhibitor of the enzyme. ?The small molecule is a competitive inhibitor of the enzyme.

Explanation / Answer

Option 3. The small molecule is an non competitive inhibitor of the enzyme. Because in non competitive inhibition, inhibitor binds to the enzyme tightly and changes its conformation but though the substrate can still bind to active site but still can not catalyze the reaction as the transition state is not achieved or stabalised enough.

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