Bacterial Genetic Exchange Scenario Reading: (See image below) In the drawing be

Question

Bacterial Genetic Exchange Scenario Reading: (See image below) In the drawing below, the bacterial cell on the left represents the donor cell (Hfr) and the bacterial cell on the right represents the recipient cell (F-). The solid line represents represents the F factor. The point marked O indicates the origin of the F factor (arrow indicates direction of replication), while the rest of the letters on the outside of the genome represent specific genes of interest to be transferred from the donor (A', B', C' and D'), and recombined with the recipient's homologous genes (A, B, C, and D). The numbers on the inside of the genome indicate the amount of time (minutes) required to completely transfer this gene via conjugation, starting at the origin of replication in the F factor. It wil take 45 minutes to transfer the entire plasmid via conjugation chromosomal DNA while the dashed line D' 33 45 ?A -A ?' C' F. Hfr Group Discussion Questions: 1. Which of the two cells carries the genetic information for the production of a pilus prior to the conjugation event: Hfr, F-, or both 2. If we allow the cells to undergo conjugation for 9 minutes, which gene/genes wi be COMPLETELY transferred successfully to the reciplent cel l?

Explanation / Answer

1. The F factor is needed for formation of pilus. The Hfr only have F factor, so Hfr can only form pilus.

2. The replication will start from the origin and it will start transferring the genes towards the arrow. If we allow the cells to conjugate for 9 minutes, the A' and B' gene will be transferred.

1. Coding strand. because during the transcription template strand is 3'->5', so the sequence of 5'->3' will be identical except T's become U in RNA.

2. 5'->3'.

3. 5'-UGG AUG ACG CGC CCC CAG TGA GGG-3'

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