Please answer ALL questions and give reason for selection of answer. Please also
ID: 218309 • Letter: P
Question
Please answer ALL questions and give reason for selection of answer. Please also number each answer so I know which question it belongs to.
Black circle is Ginkgo Leaf extract
White circle is JSE (a walnut extract)
Black triangle is Selegiline
8. Which of the three substances in the diagram above is the lea MAO-B activity? A. Ginkgo Leaf extract B. JSE (a walnut extract) C. Selegiline 9. In the experimental booklet that you had for this experiment what was the substrate used to study the effect of deprenyl on MAO-A activity? A. Clorgyline B. 5 HT C. Dopamine D. Phenylethylamine E. Benzylamine 10. You did a serial 1 in 10 dilution of a stock solution of deprenyl. For example, to make a 10-4 M. Solution from a 10-3 M stock you could take 20 ?1 of the stock and dilute it down with 180 ul of a diluent the phosphate buffer. How much diluent would you have needed to add to make a 104 M solution from the 10 M stock if you were told to first take 500 ul of the stock solution? -3 A. 4500,1 B. 5000 ul C. 180 ul again D. 2011 11. In terms of the number of radioactive counts (cpm) you would expect to have in each set of tubes in your experiment which of the following options ranks the tubes in terms of lowest number of counts to highest number of counts? A. 10 M deprenyl> 10 6 M deprenyl> 10 M deprenyl B. Control>Blank> 10 T M deprenyl C. Control> 10-11 M deprenyl> 10 M deprenyl D. 10 M deprenyl> 10 9 M deprenyl > Blank E. Blank> Control> 10 M deprenyl -5Explanation / Answer
Please find the answers below:
Answer 8: Choice C (The diagram shows that the seleginelle extract was least potent in inhibiting the MAO activity since increment in the concentration of seleginelle did not improve the MAO activity)
Answer 9: Choice C (Dopamine is generally used as a substrate to investigate the activity of MAO both in vitro and in vivo)
Answer 10: Choice A (Adding 4500+500 will give a final solution volume of 5000 and a dilution factor of 500/5000 or 1:10 which can be diluted twice again to achiev final dilution of 1:1000)
Answer 11: Choice D (A positive control gives the highest possible reading in a biochemical test, a blank gives lowest possible value for the test whereas other concentrations of the test-samples give values in increasing orders with respect to their concentrations.)
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