3. Fine stripe leaves (0. bronze leaves (b). and golden leaves (g) are 3 recessi

Question

3. Fine stripe leaves (0. bronze leaves (b). and golden leaves (g) are 3 recessive traits in com, the genes of which are linked on the same ch testcross, and the following progeny are produced: romosome. A plant heterozygous for all 3 traits is used in a 3a. Determine the order of these genes on the chromosome. [3 pts] Progeny g F B gf B G F B Gfb Number 451 134 97 436 18 119 24 g Fb GFb GfB Total: 1,365 3b. Calculate the map distances between the genes and draw out the order and distance between the genes on a genetic chromosome map. [5 pts] 3,15 3.3 3c. Determine the coefficient of coincidence (CoC) and the interference among these genes. [4 pts]

Explanation / Answer

3a) Gene order g-------- b -------- f

b) Distance between g and b = (97+86+18+24) * 100/ 1365 =16.4 mu

Distance between b and f = (119+134+18+24) * 100/ 1365 = 21.6 mu

g-----16.4 mu------b ------21.6 mu----------f

c) Coefficient of coincidence = observed DCO/expected DCO

Coefficient of coincidence = 18+24/ (0.164)(0.216) (1365) = 0.87

Interference = 1 - (observed DCO/expected DCO)

Interference = 1 - (18+24)/ (0.164)(0.216) (1365) = 0.13

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