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PLEASE EXPLAIN 11. The following DNA fragment was sequenced by the Sanger method

ID: 218232 • Letter: P

Question

PLEASE EXPLAIN

11. The following DNA fragment was sequenced by the Sanger method. The asterick indicates a fluorescent label at the 5'-end of the primer. A sample of the DNA was reacted with DNA polymerase and the following mixture of nucleotides (in an appropriate buffer): dATP, dCTP, dTTP ddGTP, and ddTTP. The ddGTP and ddTTP were included in relatively small amounts. The prodauct or products of the srqigrectnls A. *5'-primer-CAddT B. *5'-primer-CATGAGATACGTCC D. *5'-primer-ddG and *5'-primer-GddT E. *5'-primer-CAddT and *5'-primer-CATGAGAddT

Explanation / Answer

The correct answer is option C because both the products will be add as both ddGTP and ddTTP were added and first will be hauled by dd GTP and after that will be hauled by ddTTP. So, option C is the correct answer.

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