2. Given the DNA sequence: 5- CAATGTCCAAGGTGGACTGACAG-3\' i) Replicate by writin

Question

2. Given the DNA sequence: 5- CAATGTCCAAGGTGGACTGACAG-3' i) Replicate by writing the complementary strand of DNA below the given strand. Make sure to label the ends of the new strand ii) Transcribe using the DNA strand you wrote in part i) as the template strand. iiiI Translate the message you transcribed in part ii). (Careful! Look for a start codon) iv) If the A marked with an arrow in the above sequence changed to a T, what new polypeptide would be produced? (repeat steps i to ii with mutated sequence). What type of mutation is this?

Explanation / Answer

Ans:-2 (i) The Complementary sequence of the above given sequence will be.

                      3'-GTTACAGGTTCCACCTGACTGTC-5'

(ii) the mRNA of this strand 3'-GTTACAGGTTCCACCTGACTGTC-5' will be as

                        mRNA formed    5'-CAAUGUCCAAGGUGGACUGACAG-3'

(iii) start codon in this sequence 5'-CAAUGUCCAAGGUGGACUGACAG-3' is underline and bold represented thus the polypeptide will be.

   Met-ser-lys-val-asp (polypeptide)

(iv) the new mRNA produced will be..

    5'-CAAUGUCCUAGGUGGACUGACAG-3'

new peptide will be produced as:-

     Met-ser (dipeptide)

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