1. (10 points) From a kinetics experiment, Kea was determined to be 3555see1. Fo
ID: 218148 • Letter: 1
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1. (10 points) From a kinetics experiment, Kea was determined to be 3555see1. For the kinetic assay, 0.3mL of a 0.25mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 55,000 g/mole. Assume a reaction volume of 3mL. Calculate Vmax (uM the enzyme and catalytic efficiency ( in M'see') for the enzyme. The Km for the enzyme was determined to be 5.5 x 10-5M. 2. (8 points) Consider the following peptide: Lys-Tyr-Glu-His-Cys-Glu-Ala-Lys- Cys-Arg-Asp-Hi a. What is the net charge of this peptide at pH 1 (you must show work for full credit!)?Explanation / Answer
2a) Peptide - Lys- Tyr - Glu - His - Cys - Glu - Ala - Lys - Cys - Arg - Asp - His
Lys, His and Arg are positively charged amino acid so their charge at pH 1 will be +1 and Glu and asp are negatively charged amino acid their charge at pH 1 will be 0; Tyr, cys, ala are uncharged amino acid.
Charge at pH 1 - 1 + 0 + 0 + 1 + 0 + 0 + 0 + 1 + 0 + 1 + 0 + 1 = +5
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