1. Read the DNA sequence after agarose gel electrophoresis and label the ends ??
ID: 218002 • Letter: 1
Question
1. Read the DNA sequence after agarose gel electrophoresis and label the ends ??? 2. Read the DNA sequence from the norescent peaks obtained from electrophoresis in a thin capillary tube (label the ends) 1 S 10 Time 3. Which of the following is/are associated with the processes listed in the table? A. mRNA B. DNA C. cDNA D. DNA Probe E. Restriction enzymes F. DNA denaturation G. Agarose gel electrophoresis H. Chemiluminescence Process Southern blotting Northern blotting Fluorescence In Situ hybridization Component(s) 4. Classify the statements as True or false Statement True/False Mutagenesis cannot be used to study the result of gene mutation Quantitative PCR does not require a primer DNA Hybridization is conducted in a gene micro array Shortgun is refered to whole genome sequencing
Explanation / Answer
Q1. Sequence is GTACTAAATGG
Q2. Sequence is ATCAGCT
Q3.Southern hybridisation - DNA, c DNA. , Agarose gel electrophoresis, DNA denaturation, DNA probe.
Northern hybridization - mRNA , Agarose gel electrophoresis, DNA probe
In situ hybridization chemiluminiscence
Q 4. False
False
True, hybridization is done in gene array
True , shotgun term used in genome sequencing
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