Note: Respond To All Questions From Example 1 To Example 2 Below Example 1. A si

Question

Note: Respond To All Questions From Example 1 To Example 2 Below

Example 1. A simple bi-parental mating After isolating a tetracycline-resistant strain of Citrobacter freundii, a close relative of E. coli. from the environment, you want to determine if the drug resistance is transferrable (ie. found on a conjugative plasmid). To test its transferability you mix C. freundii with a lab strain of E. coli that is naturally resistant to sodium azide (ie.. resistance is chromosomally encoded). After allowing enough time for conjugation to take place, you spread the mixture onto a Petri dish of agar media. Sketch out the process taking place in this scenario How would you select for transconjugants?

Explanation / Answer

1b) Transconjugants are selected by plating on tetracycline antibiotic. The transconjugants containing tetracycline resstance genes will be able to grow in the antibiotic but the E. coli lacking tetr genes will be unable to grow in the antibiotic.

2b) Transconjugants are selected by plating them on spectinomycin and kanamycin antibiotic. Transconjugants whose pigment genes have been knocked out can be selected by plating on kanamycin antibiotic.

Related Questions

Navigate

• Browse (All)
• Browse N
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.