23. In the Anthony method of staining the bacterial capsule, which step is not i

Question

23. In the Anthony method of staining the bacterial capsule, which step is not included in order to avoid shrinking and destroying the capsule a, smear preparation b. abasic dye d. All of the above 24. In the acid fast stain, an organism such as Mycobacterium smegmatis will stain after a successful staining technique. a. Blue due to the primary stain, methylene stain b. Pink to red due to the primary stain, carbol fuchsine c. Green due to the primary stain, malachite green d. None of the above 25. The Schaeffer- Fulton method of staining utilizes to stain the endospore and to stain the vegetative cell. a. Safranin; malachite green b. Malachite green; safranin e. Methylene blue; iodine d. lodine; methylene blue 26. A common media used in a Microbiology lab is nutrient agar It is considered a media because its exact composition is not known but it contains all the necessary ingredients for most facultative organisms to grow successfully b. Selective d. Complex a. Defined 27. Rod shaped bacteria are called a. Bacilli b. Streptocooci ?. Cocci d. Spirilla 28. Some bacteria such as Clostridium form a thick- walled structure called in response to poor environmental conditions. b. Capsule c. Sheath d.Pilus 29. Nitrifying bacteria are an example of c. Chemoheterotrophs d. Photobeterotrophs a. Photosutotrophs 30. Organisms that obtain their energy by oxidizing inorganic chemical sources are called b. 31. If you carried out an experiment growing different types of microorganisms in liquid culture, which type of organism would you find congregated just a little below the liquid's surface? a. obligate aerobes e. microaerophiles d. aerotolerant bacteria b. obligate anacrobes 32. A particular agent that only inhibits the growth of bacterial cells but does not kill them is said to be while an agent that kills the bacterial cells is said to be c. Bactericidal; bacteriostatice d. Sporocidal; fungicidal b. Bacteriostatic; bactericidal 33. Which term best describes an organism that can't exist in the presence of oxygen? a. obligate acrobe b. facultative acrobe c. obligate anaerobe d. facultative anaerobe

Explanation / Answer

23) BASIC DYE, in the anthony method of positive capsule staining smear preparation on a non heat fixed bacteria is done,so that the acteria takes up the primary stain of crystal violet.

24) In acid fast stain , an organism such as mycobacterium will stain BLUE DUE TO THE PRIMARY STAIN, METHYLENE STAIN, after succesful staining technique.

25) The schaeffer faulten method of staining utilizes, MALACHITE GREEN to stain the endospore and SAFRANIN to stain the vegetative cell.

26) A coomon media used in microbiology lab is nutreint agar. It is considered a COMPLEX media because its exact composition is not known, but it contains all ingredients for most facultative growth of microorganisms.

27) Rod shaped bacteria are called BACILLI

28) Some bacteriumm such as Clostridium form a thick CAPSULE in response to poor environmental conditions.

29) Nitrifying bacteria are examples of CHEMOAUTOTROPHS OR CHEMOLITHOTROPHS.

30) Organisms that obtain energy by oxidizing inorganic chemical sources are called as CHEMOHETEROTROPHS.

32) A particular agent that only inhibits the growth of bacterial cells but does not kill them is said to be BACTERIOSTATIC while an agent that kills the bacterial cells is said tro be BACTERICIDIAL.

33) OBLIGATE ANAEROBES are the organisms that cant exist in presence of oxygen as they get poisoned by oxygen.

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