iew canvas.sta.ndu added to the 3 D Question 16 3.5 pts After a decades of work,
ID: 217560 • Letter: I
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iew canvas.sta.ndu added to the 3 D Question 16 3.5 pts After a decades of work, Dr. Octopus has built a worm hole into the Harry Potter universe and retrieved a small amount of Felix Felicus, an powerful magical serum which makes the user lucky. Dr. O discovers that the active ingredient in the serum is a protein similar to that produced by four-leaf clovers (4-LC). He identifies a human homolog for this gene, and predicts that it will be highly expressed in children, and teenagers (who he believes to be the most lucky) compared to a grumpy scientist control. Which of the following experiments will NOT help him: O PCR on cDNA from tissue samples O western blot to examine protein expression in tissue samples O In situ hybridization to examine mRNA expression in tissue samples O PCR on DNA from tissue samples. Previous Next MacBook ProExplanation / Answer
The Answer is Option No. 1: PCR on cDNA from tissue samples.
Doctor Octopus has identified the human homolog of the gene of Four-leaf clovers(whose protein is similar to Felix Felicus), For homologous recombination of the gene in the human(children and teenagers), Dr. Octopus must incorporate the original DNA of 4-LC which transcribed primary transcript, not the modified cDNA which is generated from the mRNA of 4-LC by Reverse Transcription.
cDNA is the complementary DNA which is generated from mRNA. mRNA of a species is processed from primary transcript by Post-Transcriptional Modification process. Primary transcript is the product of transcription from DNA. Primary transcript contains both Introns and Exons whereas mRNA contains only Exons. That means if cDNA is being generated from mRNA, that will only contain the DNA parts of Exons, the parts of Introns won't be present on the cDNA but for homologous recombination process, the DNA sequence must same as the homologs. The Introns and exons part both should be present on the DNA, that means there is no possibility of recombination occurrence for the cDNA. Cause of that PCR on cDNA from plant tissue have no need for this experiment.
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