3] For the figure below, answer the following question: The cells were treated w

Question

3] For the figure below, answer the following question: The cells were treated with a drug TTO in the doses ndcated 0%·control). Diamtheraphedre utnterm of all you now about growth curves (Make sure to discuss phases, growth rate constants Growth Rate of NIH-3T3 Cells 50.000 200.000 150,000 000% 50.000 Time (Day 41 This assay was generated to measure the canine CPR protein (ng/ml) by optical density at 450nm using a spectrophotometer a) Using the Beer-Lamberts explain the relationship seen and explain why the line of best fit is drawn as b) You have two samples of protein that are of unknown concentration. You measure them both using a spectrophotometer, The first sample has an absorbance of 1.0 and the second sample has an absorbance of 2.0. Interpret or if possible extrapolate these values for each unknown using the standard curve applying al the rules for using a calibration/standard curve 25 0.5 20 40 60 80 100 12 1 Canine CRP cone ngimi

Explanation / Answer

4 a) Bear lambert's law states that absorbance is directly proportional to concentration so, ideally after increasing concentration absorbance (OD: optical density) should be increased in linear correlation. Here we get 5 data point and to know the best fitted to this formula we linearly fit the graph to see how many data is in the correlation of this law. only 3 data point lies in this linear fit line and other two is deviated.

3) Control cells are not treated with drug so with refernce to control all the drug concentration is ihibitor of these cells but the blue one (0.06%) (as not so much clear image it is) is the potent concentration to inhibit the cell growth in 7 days. this concentration kills 2 times more cells in compare to pink concentration (0.0--%)

Related Questions

Navigate

• Browse (All)
• Browse 3
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.