LAB6-Prelab PRE-LAB 6 The Hill Reaction Pre-laboratory Assignment Directions: is
ID: 217538 • Letter: L
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LAB6-Prelab PRE-LAB 6 The Hill Reaction Pre-laboratory Assignment Directions: is not complete at the beginning of lab, the student will be unable to participate in the lab session Questions: TYPE the answers to the following questions and turn in BEFORE lab begins. If pre-lab 1.(2pts) While doing this experiment, it will be important to keep everything on ice. Why is it important to keep everything cold? What might happen if the proteins are warmed to room temperature? 2. (2pts) While doing this experiment, you will be centrifuging (spinning") your samples at 1000xg or 10,000 rpm. What does 1000xg really mean? What does 10,0000rpm really mean? Can you convert xg to rpm? (Is rps ALWAYS 10 times the xg?) 3. (2pts) Photosynthesis is a redox reaction. In the light-reaction of photosynthesis, what molecule is oxidized? In the dark-reaction (Calvin Cycle) of photosynthesis, what molecule is reduced? 4. (2pts) In the Hill Reaction (not photosynthesis), what would you expect to happen to absorbance when the test tube is exposed to light? Would it increase, decrease, or stay the same? Why? 5. (2pts) In the Hill Reaction (not photosynthesis), what would you expect to happen to absorbance if the test tube is NOT exposed to light? Would it increase, decrease, or stay the same? Why?Explanation / Answer
1.Temprature pays an important role during performing any experiment. Low temperature decreases the enzymatic reaction. Protein too acts as an enzyme and thus increase in temperature may lead to increase in enzymatic reaction. With increase in temperature collision of molecules increases which lead to increase in collision between the enzyme and substrate.
Thus most of the experiments is carried out in cool temperature.
2. In the configuration of the sample 1000xg, g refers to the relative configural force. It refers to the configural force of the configural tubes to machine at 1000:1. This force is dependant upon the radius of the rotation of the rotor.
RPM on the other hand refrers to the revolution per minutes.
To convert g force into rpm:
g force=(rpm)2*1.118*10-5*r
where r = Rotational radius
3. Redox reactions are the reaction in which elctrons are reduced in one rection and gained in another. It is also known as oxidation reduction reaction. During oxidation loss of electrons take place while during reduction gain of electron take place.
photosynthesis is a process during which plants prepare its food in presence of sunlight, water and carbon di oxid e. Chlorophyll convert convert CO2 into sugar by redox reaction.
CO2+H2O ----------------> (CH20) +O2
At first oxidation of water took place
2H2O ---------------------->O2+4[H]
Second step involve reduction of CO2 BY H ION
4[H]+CO2 ------------------> CH2O+H2O
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