(d) You have used a phage titer procedure to determine the titer of a phage samp

Question

(d) You have used a phage titer procedure to determine the titer of a phage sample to be 100,000 PFU/ml. You now want to use this phage sample to conduct an experiment, and you need to make sure each trial of the experiment uses the same phage concentration 100 PFU/ml. Describe and/or diagram how you will use the original phage sample to prepare a diluted sample with the desired PFU/ml for your experiment. In your description / diagram, you MUST show exactly how much phage solution and how much sterile broth you need

Explanation / Answer

Since the stock has the concentration of 100,000 and required is 100 pfu/mL we need 1000 times dilution

We can start as follow

Tube 1= 1mL of stock + 9mL of diluent = 1/10 dilution

Tube 2= 1mL from tube 1 + 9mL of diluent = 1/100 dilution

Take 1mL from the tube 2 and spread it on the sterile broth 9 mL = 1/1000 dilution

So, we expect 100pfu/mL on the broth

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